Plates containing less than 96 samples should be loaded in a vertical fashion (A1, B1, C1, D1, E1, F1, G1, H1, A2, B2, etc.) Samples are to be submitted in a 96-well plate obtained from the Genomics Core.A box for samples will be on the left side of the unit on the second shelf from the top.Ĭonsiderations for 96-well Plate Submission: Place samples in the white refrigeration unit located in the hallway directly across from room 211.(Plastic bags and permanent marker are provided on a cart outside of room 215). Place samples in a plastic bag with the LIMS order number and submitting individual's name clearly written on the outside of the bag.Each tube is labeled with the last three digits of the requisition #. Label tubes with the last three digits of the requisition number on the top of the tube.įor example: Order #15759 has two sample Requisition #289573 and Requisition #289574. Each order receives an order number and each sample receives a requisition number.Recommended total amounts in the 16ul volume for DNA templates and primer:.
Total volume of the template + primer mix in the 1.5 ml tube is to be 16 ul.The Fisher Scientific tubes may be purchased from the Enzyme Freezer Program in a 500 tube per bag quantity. #05408129)1.5ml MCT Graduated tubes for sample submissions. The MUGTC currently uses Fisher Scientific (Cat. The rack used by our Tecan robotic system restricts us to accepting only tubes with specific dimensions. All samples are to be submitted in a 1.5ml tube.
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